Part:BBa_K5115003:Design

hoxU, without stop codon

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 410
  Illegal BglII site found at 488
  Illegal XhoI site found at 418
  Illegal XhoI site found at 610
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 371
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 192
  Illegal BsaI site found at 354
  Illegal SapI.rc site found at 303
  

Design Notes

Synonym mutations are performed on a site that does not conform to the regulations

Source

https://www.sciencedirect.com/science/article/abs/pii/S0360319922019899